t cell acute lymphoblastic leukemia cell line ccrf-cem  (CEM Corporation)

Journal: Scientific Reports

Article Title: Leukoreduction filter derived NK cells offer a promising source for off the shelf CAR NK cell immunotherapy

doi: 10.1038/s41598-025-97584-1

Figure Lengend Snippet: High cytotoxicity of anti-BCMA CAR LRF-NK and PB-NK cells against BCMA-expressing cells. ( A ) Flow cytometric analysis depicts CD107a expression on anti-BCMA CAR LRF-NK cells (GFP + population) post-co-culture with U266-B1 cells (BCMA + cells). ( B ) the mean percentage of CD107a expression on anti-BCMA CAR LRF-NK cells (GFP + population) after co-culture with CCRF-CEM (BCMA − cells) and K562 cell lines. ( C ) Statistical analysis indicates significantly elevated CD107a expression on anti-BCMA CAR LRF- and PB-NK cells when co-cultured with U266-B1 compared to CCRF-CEM and K562 cells. No significant difference in CD107a expression exists between anti-BCMA CAR LRF- and PB-NK cells. Data presented as Mean ± SD. (** Represents P < 0.001, and *** P < 0.0001, N = 3). LRF: Leukocyte reduction filter. PB: peripheral blood.

Article Snippet: The human BCMA + multiple myeloma cell line (U266-B1), BCMA- acute lymphoblastic leukemia cell line (CCRF-CEM), human erythroleukemic cell line (K562), and human embryonic kidney cell line (HEK293T) were procured from the Pasteur Institute (Tehran, Iran).

Techniques: Expressing, Co-Culture Assay, Cell Culture

Journal: Scientific Reports

Article Title: Leukoreduction filter derived NK cells offer a promising source for off the shelf CAR NK cell immunotherapy

doi: 10.1038/s41598-025-97584-1

Figure Lengend Snippet: Specific activation of transduced NK cells. ( A ) The baseline expression levels of CD69 (upper left) on anti-BCMA CAR LRF-NK cells, when cultured alone and CD69 expression following the co-cultivation of anti-BCMA CAR LRF-NK cells with U266-B1 (upper right) CCRF-CEM (Lower Left) and K562 (lower left) cell lines. ( B ) The mean percentage of CD69 as an early activation marker significantly increased on anti-BCMA CAR LRF/PB cells following co-cultivation with the U266-B1 compared to CCRF-CEM cell lines.

Article Snippet: The human BCMA + multiple myeloma cell line (U266-B1), BCMA- acute lymphoblastic leukemia cell line (CCRF-CEM), human erythroleukemic cell line (K562), and human embryonic kidney cell line (HEK293T) were procured from the Pasteur Institute (Tehran, Iran).

Techniques: Activation Assay, Expressing, Cell Culture, Marker

Journal: Scientific Reports

Article Title: Leukoreduction filter derived NK cells offer a promising source for off the shelf CAR NK cell immunotherapy

doi: 10.1038/s41598-025-97584-1

Figure Lengend Snippet: mRNA expression of IFN-γ ( A ) and granzyme B ( B ) in anti-BCMA CAR LRF-NK and PB-NK cells following co-culture with U266-B1 (BCMA + ), CCRF-CEM (BCMA − ), and K562 Cell Lines. The augmented expression of IFN-γ and granzyme B was evident in LRF-NK and PB-NK cells after co-cultivation with the K562 cell line, as opposed to their counterparts co-cultivated with U266-B1 and CCRF-CEM cell lines, as well as IL-2 treated NK cells. No statistically significant distinctions were discerned between LRF-NK and PB-NK cells. Conversely, Anti-BCMA CAR NK cells demonstrated a notable increase in the expression of IFN-γ and GrB after co-culture with U266-B1 compared to CCRF-CEM. The absence of significant differences between LRF and PB CAR NK cells in IFN-γ and GrB expression under identical conditions is noteworthy. Notably, a significantly heightened expression of IFN-γ and GrB in CAR NK cells compared to regular NK cells was observed after co-culture with U266-B1 cells. Data are presented as mean ± SD. (Double asterisks (**) signify P < 0.001, and triple asterisks (***) denote P < 0.0001; N = 3). LRF: leukocyte reduction filter. PB: peripheral blood.

Article Snippet: The human BCMA + multiple myeloma cell line (U266-B1), BCMA- acute lymphoblastic leukemia cell line (CCRF-CEM), human erythroleukemic cell line (K562), and human embryonic kidney cell line (HEK293T) were procured from the Pasteur Institute (Tehran, Iran).

Techniques: Expressing, Co-Culture Assay

Journal: Pharmaceutics

Article Title: Synthesis and Anticancer Activity of Novel Dual Inhibitors of Human Protein Kinases CK2 and PIM-1 †

doi: 10.3390/pharmaceutics15071991

Figure Lengend Snippet: Viability of CCRF-CEM (acute lymphoblastic leukemia), K-562 (chronic myelogenous leukemia), MCF-7 (breast carcinoma), and non-cancerous Vero cells after treatment with the tested compounds. The IC 50 values were calculated using an MTT-based assay and the following equation: Y = 100/(1 + 10^((LogIC50 − X) ∗ HillSlope)).

Article Snippet: An acute lymphoblastic leukemia ALL cell line (named CCRF-CEM) was purchased from the European Collection of Authenticated Cell Cultures (ECACC), whereas MCF-7 (hormone-dependent breast adenocarcinoma), K-562 (human chronic myelogenous leukemia), and Vero cells ( Cercopithecus aethiops kidney) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: MTT Assay